Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml
€ 57
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Manufactured in an ISO 9001 and 13485 certified facility in the USA, our buffers offer unparalleled quality for your molecular biology research.
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Λεπτομέρειες προιόντος
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Included Components | Bottle |
| Part Number | 23.105.88 |
| Item Weight | 8 ounces (226.8 grams) |
| Brand | eCon-Lab-Supply |
Who Should Buy?
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Biologists
Ideal for experiments requiring stable pH conditions, ensuring optimal enzyme activity and accurate molecular biology workflows.
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Chemists
Useful for maintaining pH in various chemical reactions, enhancing reliability in laboratory experiments and formulations.
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Healthcare labs
Essential for diagnostic procedures or assays that require precise pH levels, improving test accuracy and patient outcomes.
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Educational use
May not suit beginners as handling buffers requires some chemistry knowledge and experience for safety and effectiveness.
ΠΕΡΙΓΡΑΦΗ ΤΟΥ ΠΡΟΪΟΝΤΟΣ
Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml
Ερωτήσεις & Απαντήσεις Πελατών
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ερώτηση:
What is a Phosphate Buffer, pH 7.2 used for?
απάντηση: A Phosphate Buffer, pH 7.2 is commonly used in biological and chemical laboratories to maintain a stable pH environment essential for various experiments. This buffer solution is particularly beneficial in enzymatic reactions, cell culture, and molecular biology applications where pH fluctuation could impact the outcome. For instance, many enzymes exhibit optimal activity at specific pH levels, and using this buffer can help maintain those conditions. -
ερώτηση:
How do you prepare a Phosphate Buffer, pH 7.2?
απάντηση: To prepare a Phosphate Buffer, pH 7.2, a precise mixture of disodium hydrogen phosphate (Na2HPO4) and monosodium phosphate (NaH2PO4) is required. This involves dissolving a calculated amount of each salt in distilled water, and adjusting the pH to 7.2 using a pH meter. This method is crucial for applications requiring accurate pH values, such as biochemical assays or cell culture media preparations. -
ερώτηση:
What are the advantages of using a Phosphate Buffer?
απάντηση: Phosphate Buffers are widely favored due to their ability to maintain stable pH levels with minimal changes upon dilution or during ionic strength variations. They are effective in a physiological range, making them ideal for biochemical reactions that mimic in vivo conditions, such as protein purification and cellular experiments. Additionally, they exhibit low absorbance at UV wavelengths, making them suitable for spectrophotometric analysis. -
ερώτηση:
Can I use Phosphate Buffer for cell culture?
απάντηση: Yes, Phosphate Buffer, pH 7.2 is frequently used in cell culture applications. It helps maintain the physiological pH that is crucial for cell viability and function. In cell culture media, this buffer plays a vital role in providing the stability needed for optimal cell growth and productivity, allowing researchers to conduct experiments without adverse effects from pH fluctuations. -
ερώτηση:
Is Phosphate Buffer suitable for protein assays?
απάντηση: Absolutely, Phosphate Buffer, pH 7.2 is highly suitable for protein assays. Many analytical methods, such as enzyme-linked immunosorbent assays (ELISA) or spectrophotometric analyses, rely on a stable pH environment to ensure accurate results. The buffer's ability to maintain consistent pH levels helps ensure that the proteins retain their biological activity and structural integrity during analysis. -
ερώτηση:
What should I consider when storing Phosphate Buffer?
απάντηση: When storing Phosphate Buffer, pH 7.2, it's important to keep it in a tightly sealed container away from direct light and temperature extremes. Exposure to air may lead to contamination or evaporation, altering its pH. Proper storage ensures that the buffer remains effective for laboratory use, especially for sensitive experiments requiring pH control. -
ερώτηση:
Can Phosphate Buffer be used in chemical synthesis?
απάντηση: Yes, Phosphate Buffer, pH 7.2 can be utilized in various chemical synthesis processes. It can help in maintaining the desired pH during reactions involving acid-base chemistry, where a stable pH is crucial for the desired outcomes. This buffer is often employed in reactions such as esterification or polymerization, where pH stability influences the reaction rate and mechanism. -
ερώτηση:
Is Phosphate Buffer compatible with all biochemical reactions?
απάντηση: While Phosphate Buffer is versatile, it's not suitable for all biochemical reactions. Some reactions may be sensitive to phosphate ions or require a different pH range. For instance, reactions involving magnesium-dependent enzymes may necessitate using alternative buffering systems. Therefore, it's essential to evaluate the specific requirements of your experiment to ensure compatibility. -
ερώτηση:
What is the concentration of Phosphate Buffer, pH 7.2?
απάντηση: The Phosphate Buffer, pH 7.2 is available in a concentration of 1.0 M, which indicates that it contains 1 mole of phosphate ions per liter. This concentration is often preferred for laboratory applications because it provides effective buffering capacity and maintains stability against pH changes during biochemical processes. Higher concentrations might be too strong for some applications, while lower concentrations could compromise the buffering ability. -
ερώτηση:
Where can I buy Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml?
απάντηση: You can easily purchase Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml from Ubuy in Greece. Ubuy offers a wide selection of laboratory reagents, including this buffer solution, ensuring a convenient shopping experience for your laboratory needs.
eCon Lab Supply Buffers Editorial Review
Customer Reviews & Ratings
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5 αστέρι
100%
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4 αστέρι
0%
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3 αστέρι
0%
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2 αστέρι
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1 αστέρι
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Πλεονεκτήματα
- Highly effective pH stabilization
- Pre-made for convenience
- Reliable for laboratory use
- consistent quality and performance
- Suitable for various applications
Μειονεκτήματα
- Bottle design could be improved.
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€ 57
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Χαρακτηριστικά & Πλεονεκτήματα
- Comprehensive range of biological buffers for research and diagnostics.
- Ideal for protein research, molecular biology, and characterization.
- Manufactured in USA in ISO 9001 and 13485 certified facility.
- Produced under stringent Good Manufacturing Practices.
- Quality control tests conducted in certified 14644 clean room conditions.
- Perfect for immunochemical staining and preserving living tissues.
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